Putative in vitro expressed gene fragments unique to Mycobacterium avium subspecies paratuberculosis.
نویسندگان
چکیده
By a suppression subtractive hybridization based method, nine novel Mycobacterium avium subsp. paratuberculosis (M. paratuberculosis) fragments of between 318 and 596 bp have been identified and characterized. Database search revealed little or no similarity with other mycobacteria. The uniqueness and diagnostic potential of seven of these fragments in relation to M. paratuberculosis closest relative Mycobacterium avium subsp. avium (M. avium) was confirmed by species-specific PCR and Southern blot. Furthermore, RT-PCR indicated that eight of the nine fragments originate from areas of the genome that are expressed in vitro.
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ورودعنوان ژورنال:
- FEMS microbiology letters
دوره 214 2 شماره
صفحات -
تاریخ انتشار 2002